alsD [2017-8-11 16:0:3]

acetolactate decarboxylase

Locus: BSU36000

Isoelectric point: 4.60

Molecular weight: 28.65 kDa

Protein length: 255 aa

Gene length: 765 bp

Function: overflow metabolism

Product: acetolactate decarboxylase

Essential: no

E.C.: 4.1.1.5

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → Overflow metabolism
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 3,708,799 → 3,709,566

The protein

Catalyzed reaction/ biological activity

• (2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO₂ (according to Swiss-Prot)

Protein family

• alpha-acetolactate decarboxylase family (according to Swiss-Prot)

Modification

• phosphorylated on ser/ thr/ tyr PubMed, in vitro phosphorylated by PrkC on Ser-88 PubMed

Expression and Regulation

Operons

• Genes: alsS-alsD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • AlsR: activation, in the presence of acetate PubMed, in AlsR regulon
  • Rex: repression, if the ratio NADH2/NAD is high PubMed, in Rex regulon
  • stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript PubMed, in stringent response

  Regulation

  • induction by acetate (AlsR) PubMed
  • expression is heterogeneous PubMed
  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

Biological materials

Expression vector

• for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP822, available in Jörg Stülke's lab, PubMed

References

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